Synthetic Plant Glycans and Their Application in Cell Wall Biology
Max-Planck-Institut für Kolloid- und Grenzflächenforschung, Am Mühlenberg 1, 14476, Potsdam, GER
Plant cells are surrounded by a polysaccharide-rich matrix that constitutes the cell wall of all higher plants. These structurally complex polysaccharides provide an important resource for food, industrial biomaterials, and the generation of bioenergy. Well-defined molecular tools mimicking natural oligo- and polysaccharides have great potential for studying structure and function of cell wall glycans and their interactions.
We have prepared oligosaccharides derived from different cell wall polysaccharides using automated glycan assembly. The assembled glycans were subsequently printed as microarray to characterize antibodies that recognize plant cell wall glycans. Furthermore, we prepared a series of xylan polysaccharides with perfectly regular patterns of arabinose substitution by enzymatic polymerization of chemically synthesized oligosaccharide building blocks. Due to the defined nature of the polysaccharide substitution patterns, specific properties, such as the ability to adsorb to cellulose, were observed for particular members of the prepared arabinoxylan collection.